WebAnswer (1 of 3): Why is agar heated before gel electrophoresis? TLDR: The heat energy increases the entropy in the agar molecules causing it crosslink to the gelling monomer units to form a sieve.The degree of this cross-linking decreases the porosity of the Gel and is directly propotional to th... WebThe microwave oven is very convenient for melting agar for viable cell counts. Composite data of four microwave ovens indicated that melting time for 50 ml of agar per bottle was about 1 min for one bottle, 1.5 min for two bottles, …

Cartwheel pattern for the swabbing of the bacterial strains tested …

WebEither add 0.5uL of 10mg/mL EtBr to 50mL of gel after boiling or add 5uL to 500mL of buffer and stain after running. Stain 10 minutes, rinse with buffer, then destain 10 minutes. You … http://www.protocol-online.org/biology-forums-2/posts/25207.html my cat breed

Ethidium bromide and SYBR Green I enhance the ... - ScienceDirect

WebGelRed® is an ultra sensitive, extremely stable and environmentally safe fluorescent nucleic acid dye designed to replace the highly toxic ethidium bromide (EtBr) for staining dsDNA, ssDNA or RNA in agarose gels or polyacrylamide gels. Safer than EtBr: non-mutagenic and non-hazardous for disposal Much more sensitive than EtBr and SYBR® Safe Web1) When you make you agar stock, don’t make full bottles that leave no room for the heated liquid to expand. Fill bottles halfway. 2) Take the lid off the bottle before microwaving; it … WebPlate 1 volumes (~100µL) of the mixture onto one LB agar plates supplemented with appropriate antibiotic: (for us Chloramphenicol 35 µg/mL or Tetracycline 12.5 µg/mL or both Chloramphenicol 35 µg/mL and Tetracycline 12.5 µg/mL depending on the clone) Incubate plates at 37°C overnight; off guard electrical coating