Web14 mai 2016 · For animals that are productively infected with MCMV, evaluate the numbers and/or percentage of Ly49H + NK cells in the blood or specific organs (e.g., spleen, liver, or lungs) at various time points post-infection. Typically, time points of interest include day 7 (peak of the effector phase), day 14, and day 21 (the contraction phase), and day ... WebInvitrogen Anti-Ly-49H Monoclonal (3D10), eBioscience™, Catalog # 13-5886-82. Tested in Flow Cytometry (Flow) applications. This antibody reacts with Mouse samples. ... Ly49H is a member of the C-type lectin Ly-49 multigene family of receptors found on natural killer cells. Ly49H, like Ly49D, lacks the characteristic ITAM (immunoreceptor ...
Anti-Ly-49H mAb redirects lysis of human Daudi cells. B6
WebRequest Bulk Quote. Description. Ly49H, also known as Cmv1 and Klra8, is a 28.3 kD protein. It is expressed on NK cells (but not on NKT cells) from C57BL/6 mice (but not … Web22 sept. 2024 · To investigate the role of T and B cells in adaptive NK cell responses against MCMV infection, we compared Ly49H + NK cell expansion in wild-type (WT) mice versus Rag2 −/− mice, which lack adaptive lymphocytes. Whereas WT mice exhibited a robust NK cell response, peaking ∼7 days post-infection (PI), Rag2 −/− mice showed a diminished … pdx fire protection
Tracking Effector and Memory NK Cells During MCMV Infection
Web15 nov. 2004 · Ly49H(+) NK cells play a critical role in innate antiviral immune responses to murine CMV (MCMV). Ly49H(b6) recognition of MCMV-encoded m157 on infected cells activates natural killing required for host resistance. We show that mAb 3D10 (anti-Ly49H) recognizes comparable subsets of NK cells from New … WebLy49H-positive (Ly49H ) NK cells preferentially proliferated during early MCMV infection, as determined by bromodeoxyu-ridine (BrdU) incorporation. BrdU Ly49H NK cells were still ... (17A2), anti-Ly49H (3D10), anti-CD69 (H1.2F3), anti-CD11b (M1/70), and anti-CD49b (DX5) were purchased from eBioscience (San Diego, WebSupplemental Experimental Procedures Parabiosis Parabiotic mice were generated as reported (Franklin et al., 2014) using 5- to 6-week-old age- and weight- pdx gold pump