Web(A) EA.hy926 cells were cultured in serum-free DMEM, serum-free DMEM with 100 ng/ml CHI3L1, serum-free DMEM with 100 ng/ml IL-8 and serum-free DMEM with 50 ng/ml VEGFA. The cells incubated with serum-free DMEM alone were used as a control (magnification, ×40). Web(Fig. 1), the EA.hy926 cells were cultured for 6 days in DMEM culture medium with either 5.5 or 25 mM D-glucose (representing normal and high-glucose conditions, respec-tively). During cell culture the medium was changed after 3 days. This medium was supplemented with 10 % FBS, 1%L-glutamine, 2 % HAT, and 1 % penicillin/streptomycin
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WebEA.hy926 cell line was purchased from ATCC (ATCC CRL-2922). EA.hy926 cells were grown in DMEM medium supplemented with 10% fresh bovine serum in a humidified atmosphere containing 5% CO 2 at 37 °C. Cultured cells without treatment served as controls. Confocal fluorescence imaging methods WebApr 1, 2024 · Human EA.hy926 vascular endothelial cells were cultured in DMEM/F-12 medium and human peripheral blood monocyte (THP-1) cells were cultured in RPMI-1640 medium (Procell, China) with 10% fetal bovine serum (v/v, FBS), 1% penicillin (v/v) and 1% streptomycin (v/v). Both of the cells were grown in a 37 °C cell culture incubator (PHCbi, … litcharts farmers bride
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WebMar 22, 2016 · EA.hy926 cells were seeded in a 96-well plate (3 × 10 3 cells in 200 μl of growth media per well). After 1, 2, or 3 days the media were replaced with UC-MSC-conditioned media, UC-MSC-conditioned media supplemented with 200 ng/ml anti-VEGF antibody (ab9570; Abcam), or fresh growth media (control wells). WebAug 22, 2016 · Human endothelial cell line (EA.hy926) was grown in a complete DMEM/F-12 medium (Gibco; Grand Island, NY), which was supplemented with 10% heat-inactivated fetal bovine serum (FBS) … Web上海慧颖生物科技有限公司专注于Hs578Bst细胞、ND7/23细胞、bel-7407细胞、EA.hy926细胞、Gibco10099-141C胎牛血清生产研发,产品囊括 ... litcharts facing it by yusef komunyakaa